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Title: INSILICO FUNCTIONAL ANNOTATION OF HYPOTHETICAL ORFs IN HUMAN CHROMOSOME2

Authors: Dr. Sivashankari Selvarajan1 and Dr. Piramanayagam Shanmughavel 2

Topic: Agriculture & Biotechnology

Abstract:

The high-throughput genome projects have resulted in a rapid accumulation of genome sequences for a large number of organisms and large number of genes with unknown function (Hypothetical). To fully realize the value of the data, scientists need to identify proteins encoded by these genes and understand how these proteins function in making up a living cell. With experimentally verified information on protein function lagging far behind, computational methods are needed for reliable and large-scale functional annotation of proteins. Functional annotation is the process of identifying for a given gene its biological function, interaction with other elements, involvement in metabolic pathways, and any other piece of information that helps in understanding when and how a gene influences the overall system. On the other hand, many Biological Processes and Disease mechanisms are still unknown due to lack of knowledge about the function of the Hypothetical genes in Human. Once its function is revealed the so called hurdle of unknown mechanism of the Human Genome can be mastered. Hence, the present study aims to use computational approaches to annotate the function of hypothetical genes in Chromosome 2 of Human. The annotation of the hypothetical genes in human chromosoem2 was done both at the nucleotide and protein level. Among the 41 uncharacterized hypothetical genes in Human chromosome 2, the functions of 27 of them were successfully annotation. Further, experimental validation is essential to confirm the predicted function.

Keywords: Annotation, Chromosome2, Function, Hypothetical genes

 

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    COMMENT - 1

  • Dr. AJIT V PANDYA (Viewer) 20th Nov 2015 - 11:17 AM
    Good morning from Dr. Ajit V Pandya form Ahmedabad ..India
    My questions / suggestion (s) are as follows…my email id is ajit1sujit@gmail.com
    Watsapp no. 91 8905599852

    1. THIS TYPE OF STUDY IS VERY DIFFICULT AND MUST BE APPRECIATED.......
    2...IT INCLUDE LOTS OF TECHNIQUES AND LONG PERIODS.....ISNT IT ?

  • COMMENT - 2

  • Dr. AJIT V PANDYA (Viewer) 22nd Nov 2015 - 12:17 PM
    good morning....
    whats progress.....

  • COMMENT - 3

  • Dr. AJIT V PANDYA (Viewer) 22nd Nov 2015 - 10:15 PM
    i have basic questions.....1.. chromosome and gene are in pair ? from that which is functional and what will determine which is active ?
    2...how and what makes nucleosomal opening and again clossing as such just in few mins /or / secconds...
    .for replication and transcription ?

  • COMMENT - 4

  • S. Sivashankari (Author) 26th Nov 2015 - 1:32 PM
    1. Thanks for the comments...This work is based on insilico analysis using BLAST2GO...
    2. The gene is the functional and nucleosomal opening/closing takes place by one of the following ways:
    Nucleosomal sliding enabling RNA Polymerase to bind to the promoter region
    Action of DNAse I on the chromatin of the promoter/enhancer
    Histone tails are acetylated to loose its binding with chromatin to enable gene expression
    Enhancers/Silencers signal open/close of the histone domain

  • COMMENT - 5

  • Sincy Joseph (Viewer) 27th Nov 2015 - 5:59 PM
    Article is good and in broad field ... good work...congrats...