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Title: Analysis of correlation of CYR61 and MTHFR Gene Polymorphism in Legg-Calve-Perthes disease.

Authors: Ajai Singh, Sabir Ali, Syed Rizwan Hussain, Vineet Kumar, Abbas Ali Mahdi, Rajeshwer Nath Srivastava

Topic: Clinical Research

Abstract:

Background:

Legg-Calve-Perthes disease (LCPD)is one of the most common causes of paediatric femoral head osteonecrosis. Besides the other known etiological aspects, till now genetic aspect has not been studied extensively.  The present study was aimed to find the association of genetic polymorphism of CYR61 and MTHFR gene with the LCPD.

 

Materials andMethods:

Single Nucleotide Polymorphisms (SNPs) analysis of the CYR61 and MTHFR genes in 41LCPDpatients and 110 healthy controlswere genotyped in this hospital-basedstudy by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP).

 

Results:

The frequency of CYR61gene homozygous mutant GG polymorphism was not significant in LCPD patients when compared with controls.MTHFRC677T homozygous mutant TT polymorphism was significant in LCPDpatients as compared to controls.

 

Conclusions:

The present study showed asignificant association of T allele of MTHFR C677T polymorphismwith LCPD and may be regarded as a risk factor to develop the LCPD in North Indian patients.

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    COMMENT - 1

  • Dr Bashir Adam Yakasai (Viewer) 21st Nov 2015 - 1:38 AM
    A good but rather a complex study. A geneticist needs to analyse and comment on it.

  • COMMENT - 2

  • AJAI SINGH (Author) 21st Nov 2015 - 11:44 AM
    Dear Dr. Bashir,
    Thnx for the comment.
    This article belongs to translation research, bridging the gap between the scientist at lab and clinician at bed side. To explore the new treatment motility, a clinician must work on basic etiopathogenesis of a disease. With the same aim we have presented our work.

    Ajai Singh

  • COMMENT - 3

  • AJAI SINGH (Author) 21st Nov 2015 - 11:50 AM
    Dear Dr. Bashir,
    Thnx for the comment.
    This article belongs to translation research, bridging the gap between the scientist at lab and clinician at bed side. To explore the new treatment motility, a clinician must work on basic etiopathogenesis of a disease. With the same aim we have presented our work.

    Ajai Singh
    • Madhumati Varma (Viewer) 25th Nov 2015 - 12:16 AM
      Good study Legg-Calve-Perthes disease (LCPD)
      Dr Madhumati Varma

  • COMMENT - 4

  • AJAI SINGH (Author) 25th Nov 2015 - 8:13 AM
    Dear Dr. Madhumati Varma,
    Thanx for your appreciation.
    Ajai Singh

  • COMMENT - 5

  • AJAI SINGH (Author) 25th Nov 2015 - 8:32 AM
    Dear Dr. Madhumati Varma,
    Transnational research is need of hour bridging gap between knowledge at lab and its clinical implications.
    Thnx
    Ajai Singh

  • COMMENT - 6

  • Diana Thamby Ebenezer (Viewer) 26th Nov 2015 - 6:48 PM
    Dear Sir,

    This is a good study looking at the molecular aspect on an Important clinical condition.
    What is your Recommendation based on your study?
    Are you sure whether this is a case control study? If yes, How the controls were selected? Any matching was done to make them comparable and negating confounding factor(s) if any?

  • COMMENT - 7

  • AJAI SINGH (Author) 26th Nov 2015 - 7:06 PM
    dear Diana, thanx for your interest. its not a case control study rather a cohort study (pilot study) which was aimed to study the molecular basis of Perthes disease. its shows significance of translational research. as far as recommendations are concered, basic aim of this pilot study was to prepare a reseach question for bigger study. i am sure our paper will stimulate many workers like you to start new reseach in this domain.

  • COMMENT - 8

  • AJAI SINGH (Author) 26th Nov 2015 - 7:10 PM
    To All, it is a very significant and burning topic. i wish to do more research in this area bt our resources are very limited. if any one interested and have fund, plz contact me at- as29762@gmail.com
    thanks

  • COMMENT - 9

  • Vinod Ramesh Gyanchandani (Viewer) 27th Nov 2015 - 4:28 PM
    Dear Dr. Singh,

    I congratulate you for presenting data on such a complex issue; result of this article says that: The frequency of CYR61 gene homozygous mutant GG polymorphism was not significant in LCPD patients when compared with controls. MTHFRC677T homozygous mutant TT polymorphism was significant in LCPD patients as compared to controls; do you really think that the data presented by you is sufficient enough or more researjust wanted to drag your attention to know re-confirm with the result part which

  • COMMENT - 10

  • Vinod Ramesh Gyanchandani (Viewer) 27th Nov 2015 - 4:30 PM
    In continuation to above comments: do you really think that the data presented by you is sufficient enough or more research is required to confirm this tall claim.

  • COMMENT - 11

  • AJAI SINGH (Author) 27th Nov 2015 - 5:00 PM
    dear Vinod, thanx for your interest and appreciation. its really a complex issue. in fact you are right the data is large enough to draw a convincing conclusion but we have never claimed that. it was just a pilot study, though we have examined both cases and control. this number is good enough to establish a research hypothesis which will be the basis of futher studies.

  • COMMENT - 12

  • AJAI SINGH (Author) 27th Nov 2015 - 5:06 PM
    dear Vinod, in continuation with above, authors never said that we are making " tall claims". very humbly and respectfully we suggest that on basis of above observations and conclusions, more studies with large sample size and if possible multicentric studies are required. this aspect related with Perthes disease was never studies (best of my knowledge) so we are very happy to share our early results. thanx