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Title: Evaluation of Anti-oxidant Activity and cytotoxicity of Strobilanthes cuspidata (Benth.) T. Anderson.

Article Number: EC2/2015/201

Authors: Sincy Joseph, Lekha Kumar, V Narmatha Bai.

Topic: Agriculture & Biotechnology


Strobilanthes cuspidata (Benth.) Anderson (Acanthaceae) are used by the tribal people of Nilgiri hills for joint pain and inflammation. A detailed study on the natural products present in endemic medicinal plants as well as pharmacological aspects will pave way to discover new drugs. Hence the present study was attempted to assess the biological activities of the crude extracts by antioxidant and cytotoxic assays. The 70% hydro-ethanolic extract of S. cuspidata was prepared to study DPPH radical scavenging activity, hydrogen peroxide scavenging activity, metal chelating activity, total antioxidant activity and in vitro cytotoxicity was assessed in Vero cell lines (African, Green monkey Kidney and Normal). The results showed that the plant extract of   S. cuspidata possesses antioxidant and free radical scavenging properties. Moreover in vitro cytotoxic activity of the alcoholic leaf extract was assessed in Vero cell lines (African, Green monkey Kidney, normal). The percentage of growth inhibition was calculated using the concentration of the leaf extract to inhibit the cell growth by 50% (CTC50). The study revealed that the activities observed could be attributed to the presence of some phytochemicals which have been associated with antioxidant and cytotoxic property.


    COMMENT - 1

  • Dr. AJIT V PANDYA (Viewer) 18th Nov 2015 - 10:45 PM
    Good morning from Dr. Ajit V Pandya form Ahmedabad ..India
    My questions / suggestion (s) are as follows…my email id is
    Watsapp no. 91 8905599852

    1. dry or fresh leaves were used for anti-oxidant activity ?

    2. how anti-oxidant and inflammations are linked ?

    3....antioxidant and cytotoxic property.- are seems to be controdictory ?
    • Sincy Joseph (Author) 26th Nov 2015 - 10:32 PM
      1.The ethanolic crude extract was used for the study.
      2. ... the studies were cytotoxicity ...
      3. This is a part of research work were these activities were necessarily done to prove the plant medicinal....
      Thank you

  • COMMENT - 2

  • Dr. AJIT V PANDYA (Viewer) 25th Nov 2015 - 12:25 AM
    good morning.....
    • Sincy Joseph (Author) 26th Nov 2015 - 10:32 PM
    • Sincy Joseph (Author) 26th Nov 2015 - 10:39 PM

  • COMMENT - 3

  • C.D. Dayanand (Viewer) 25th Nov 2015 - 9:31 AM
    Dear author
    good information
    I personally feel that study would have been better if concentrates on anti inflammatory activity and toxicity.
    selectively used only leaves..... is my question
    alcoholic extract means ethonolic/methonolic/ propanolic etc need to know

    Prof Dr C.D.Dyanand
    • Sincy Joseph (Author) 26th Nov 2015 - 10:15 PM
      This portion is a part of my work ,and during phytochemical analysis the crude extract proved to be active than leaves hence evaluation was done with the extract.

  • COMMENT - 4

  • S. Sivashankari (Viewer) 26th Nov 2015 - 1:44 PM
    Dear Author,

    Greetings..Very good work...Would like to know which part of the plant was used for evaluation...
    • Sincy Joseph (Author) 26th Nov 2015 - 10:11 PM
      ...the ethanolic extract was used for evaluation...

  • COMMENT - 5

  • P.Prathyusha (Viewer) 26th Nov 2015 - 10:29 PM
    wonderful work you have mentioned only tribals.............could you please tel me which group (tribal) is using this plant
    • Sincy Joseph (Author) 26th Nov 2015 - 10:38 PM
      the tribes of nilgiris ...todas ,paniyas and irulas.

  • COMMENT - 6

  • P.Prathyusha (Viewer) 26th Nov 2015 - 10:30 PM
    what you meant by hydro-ethanolic extract?
    • Sincy Joseph (Author) 26th Nov 2015 - 10:41 PM
      i have taken 30% water and 70% ethanol hence hydroalcohol..

  • COMMENT - 7

  • P.Prathyusha (Viewer) 26th Nov 2015 - 10:32 PM
    have done any compound isolation work................? it will add weightage to your paper.........

  • COMMENT - 8

  • P.Prathyusha (Viewer) 26th Nov 2015 - 10:35 PM
    is there any active compound isolated from your plant? if so which part? any report.?

  • COMMENT - 9

  • Sincy Joseph (Author) 26th Nov 2015 - 10:43 PM
    yes have isolated 4 compounds....

  • COMMENT - 10

  • Sincy Joseph (Author) 26th Nov 2015 - 10:44 PM
    yes... from the leaves ...reports are yet be published.

    thank you..

  • COMMENT - 11

  • J.Carolin Joe Rosario (Viewer) 27th Nov 2015 - 11:48 AM
    Hello Cincy
    Article is good and broad field, you have done a lot.Congrats.

    J.Carolin, Nirmala.
    • Sincy Joseph (Author) 27th Nov 2015 - 5:12 PM
      ...thank you Carolin mam...