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Title: Biofilm: Contributing factor to drug resistance in Staphylococci in Blood stream infections

Article Number: EC2/2015/183


Topic: Medicine


Staphylococci represent the most commonly encountered blood culture isolates from ICU patients. Slime production is considered to be a significant virulence factor for some strains of Staphylococci. The importance of the role played by slime is further increased by its frequent association to reduced antibiotic susceptibility. This study was done to find out relationship of Bio-Film formation on the susceptibility pattern of the isolates. 27.6% of the isolates produced biofilm as detected by Congo Red Agar method; commonest being Staphylococcus epidermidis (53.9%). The percentage of resistance in the group which produces biofilm is always greater than the group which does not produce biofilm. Odds ratio for overall drug resistance was 2.10 (with 95% confidence interval as [1.6 – 2.6]) and with a p-value of 0.006.


    COMMENT - 1

  • Dr. AJIT V PANDYA (Viewer) 18th Nov 2015 - 2:02 PM
    Good morning from Dr. Ajit V Pandya form Ahmedabad ..India
    My questions / suggestion (s) are as follows…my email id is
    Watsapp no. 91 8905599852

    1. hospital induced diseases are increasing and also resistant develops from overdosage of antibiotics....? agree or disagree.....give reeason please

    2. what is composition of biofilm...? please explain...

  • COMMENT - 2

  • Surya Narayanan Sethumadavan (Viewer) 19th Nov 2015 - 4:05 PM
    It is mentioned in your article 460 patients were studied. where and how were they selected and how long were they studied.

  • COMMENT - 3

  • Sandeep T (Author) 22nd Nov 2015 - 6:45 PM
    460 patients were selected using bates criteria for sepsis / blood stream infection

  • COMMENT - 4

  • Sandeep T (Author) 22nd Nov 2015 - 6:49 PM
    Development of resistance is due to various factors. one of the determinants is exposure to antibiotics. those organisms which are exposed to inappropriate levels of antibiotics, under the influence of selection pressure tend to mutate and acquire resistance. this exposure to antibiotics often happens in a health care setting - hospitals. hence resistance is seen in hospital isolates more frequently than in community strains of bacteria

  • COMMENT - 5

  • Sandeep T (Author) 22nd Nov 2015 - 6:52 PM
    i have not analyzed the composition of biofilms since it was my study objective. but literature states that staphylococcal biofilms are made up of polysaccharides, proteins and DNA from dead bacteria.

  • COMMENT - 6

  • AJAI SINGH (Viewer) 24th Nov 2015 - 11:50 AM
    Dear Dr. Sandeep,
    Should mention the ethical clearance in the method section.
    Should mention the some demographic characteristic of the patients in result section.
    Ajai Singh

  • COMMENT - 7

  • C.D. Dayanand (Viewer) 25th Nov 2015 - 9:19 AM
    Dear author

    Information is good.nice research area for progression
    provide to justify with a any reference article published in PUBMED/SCOPUS to authenticate biofilm alone is responsible for resistance.

    Prof Dr.C.D.Dayanand

  • COMMENT - 8

  • Sandeep T (Author) 25th Nov 2015 - 11:03 AM
    with respect to ethical issues, the study was conducted on bacteria isolated from patient samples received in the laboratory. though this study did not warrant an ethical clearance, the protocol was presented before the institutional ethical committee and clearance was taken.

  • COMMENT - 9

  • Amitabha Ray (Viewer) 25th Nov 2015 - 10:46 PM
    Perhaps the authors will want to investigate the biochemical nature of bio-film which is formed, and if there is any difference between different geographical parts of the country.

  • COMMENT - 10

  • Dr. AJIT V PANDYA (Viewer) 26th Nov 2015 - 11:46 AM
    good morning.......just one day more ......good progressing ........very informative and systematic .........worldclass conference......
    26 th Nov 2015 - 12:24 AM.....nice work....